ChIP analysis of sheared chromatin from 10? U2OS cells using GTX60359 MBD1 antibody - ChIP grade. Beads only were used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the MLH1 gene (used as a positive control) and CDC6 gene (used as a negative control). This figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
ChIP analysis of sheared chromatin from 10? U2OS cells using GTX60359 MBD1 antibody - ChIP grade. Beads only were used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the MLH1 gene (used as a positive control) and CDC6 gene (used as a negative control). This figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
MBD1 antibody - ChIP grade
GTX60359
ApplicationsWestern Blot, ChIP Chromatin ImmunoPrecipitation, ELISA
Product group Antibodies
ReactivityHuman, Mouse
TargetMBD1
Overview
- SupplierGeneTex
- Product NameMBD1 antibody - ChIP grade
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500. ELISA: 1:1,000. ChIP assay: 1-5 microg. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot, ChIP Chromatin ImmunoPrecipitation, ELISA
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.7 mg/ml
- ConjugateUnconjugated
- Gene ID4152
- Target nameMBD1
- Target descriptionmethyl-CpG binding domain protein 1
- Target synonymsCXXC3; CXXC-type zinc finger protein 3; methyl-CpG-binding domain protein 1; PCM1; protein containing methyl-CpG-binding domain 1; RFT; the regulator of fibroblast growth factor 2 (FGF-2) transcription
- HostRabbit
- IsotypeIgG
- Protein IDQ9UIS9
- Protein NameMethyl-CpG-binding domain protein 1
- Scientific DescriptionThe protein encoded by this gene is a member of a family of nuclear proteins related by the presence of a methyl-CpG binding domain (MBD). These proteins are capable of binding specifically to methylated DNA, and some members can also repress transcription from methylated gene promoters. This protein contains multiple domains: MBD at the N-terminus that functions both in binding to methylated DNA and in protein interactions; several CXXC-type zinc finger domains that mediate binding to non-methylated CpG dinucleotides; transcriptional repression domain (TRD) at the C-terminus that is involved in transcription repression and in protein interactions. Numerous alternatively spliced transcript variants encoding different isoforms have been noted for this gene.[provided by RefSeq, Feb 2011]
- ReactivityHuman, Mouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203