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Lane 1: MCF-7 cell lysates; Lane 2: A549 cell lysates probed with MC1 Receptor Polyclonal Antibody, Unconjugated (bs-23517R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: MCF-7 cell lysates; Lane 2: A549 cell lysates probed with MC1 Receptor Polyclonal Antibody, Unconjugated (bs-23517R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: MCF-7 cell lysates; Lane 2: A549 cell lysates probed with MC1 Receptor Polyclonal Antibody, Unconjugated (bs-23517R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

MC1R Polyclonal Antibody

BS-23517R
Bioss Antibodies
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetMC1R
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    MC1 Receptor Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID4157
  • Target name
    MC1R
  • Target description
    melanocortin 1 receptor
  • Target synonyms
    alpha melanocyte stimulating hormone receptor; CMM5; MC1-R; melanocyte-stimulating hormone receptor; melanotropin receptor; MSH-R; SHEP2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ01727
  • Protein Name
    Melanocyte-stimulating hormone receptor
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MC1 using anti-MC1 antibody (A00855-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: PC12 whole cell lysates, Lane 2: HEPA1-6 whole cell lysates, Lane 3: HELA whole cell lysates, Lane 4: A375 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MC1 antigen affinity purified polyclonal antibody (Catalog # A00855-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MC1 at approximately 35KD. The expected band size for MC1 is at 35KD.
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