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MC2R Polyclonal Antibody

RD76346A
Reddot Biotech
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetMC2R
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Overview

  • Supplier
    Reddot Biotech
  • Product Name
    MC2R Polyclonal Antibody
  • Delivery Days Customer
    5
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID4158
  • Target name
    MC2R
  • Target description
    melanocortin 2 receptor
  • Target synonyms
    ACTHR, adrenocorticotropic hormone receptor, ACTH receptor, MC2 receptor, adrenocorticotropin receptor, corticotropin receptor, melanocortin 2 receptor (adrenocorticotropic hormone)
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    MC2R encodes one member of the five-member G-protein associated melanocortin receptor family. Melanocortins (melanocyte-stimulating hormones and adrenocorticotropic hormone) are peptides derived from pro-opiomelanocortin (POMC). MC2R is selectively activated by adrenocorticotropic hormone, whereas the other four melanocortin receptors recognize a variety of melanocortin ligands. Mutations in MC2R can result in familial glucocorticoid deficiency.
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MC2 receptor using anti-MC2 receptor antibody (A02306-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. lane 1: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MC2 receptor antigen affinity purified polyclonal antibody (Catalog # A02306-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MC2 receptor at approximately 39KD. The expected band size for MC2 receptor is at 34KD.
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