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MCCC2 / MCCB Antibody

LS-C816672
LifeSpan BioSciences
ApplicationsImmunoFluorescence, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetMCCC2
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    MCCC2 / MCCB Antibody
  • Delivery Days Customer
    14
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    ImmunoFluorescence, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    ICC (1:50 - 1:200), IF (1:50 - 1:200), IHC-P (1:50 - 1:200) Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID64087
  • Target name
    MCCC2
  • Target description
    methylcrotonyl-CoA carboxylase subunit 2
  • Target synonyms
    MCCB, MCCCbeta, methylcrotonoyl-CoA carboxylase beta chain, mitochondrial, 3-methylcrotonyl-CoA carboxylase 2, 3-methylcrotonyl-CoA carboxylase non-biotin-containing subunit, 3-methylcrotonyl-CoA:carbon dioxide ligase subunit beta, MCCase subunit beta, biotin carboxylase, methylcrotonoyl-CoA carboxylase 2 (beta), methylcrotonoyl-Coenzyme A carboxylase 2 (beta), non-biotin containing subunit of 3-methylcrotonyl-CoA carboxylase, testicular secretory protein Li 29
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MCCC2 using anti-MCCC2 antibody (A06515-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat kidney tissue lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MCCC2 antigen affinity purified polyclonal antibody (Catalog # A06515-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MCCC2 at approximately 61 kDa. The expected band size for MCCC2 is at 61 kDa.
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Lane 1: Mouse kidney lysates; Lane 2: DU145 cell lysates probed with MCCC2 Polyclonal Antibody, Unconjugated (bs-18719R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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Immunohistochemistry analysis in human kidney and skeletal muscle tissues using Anti-MCCC2 antibody. Corresponding MCCC2 RNA-seq data are presented for the same tissues.
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