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Immunohistochemistry of paraffin-embedded Human lung cancer tissue using MCM2 Polyclonal Antibody at dilution 1:50
Immunohistochemistry of paraffin-embedded Human lung cancer tissue using MCM2 Polyclonal Antibody at dilution 1:50
Immunohistochemistry of paraffin-embedded Human lung cancer tissue using MCM2 Polyclonal Antibody at dilution 1:50

MCM2 Polyclonal Antibody

E-AB-14211
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    MCM2 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications Supplier
    ELISA IHC
  • Certification
    Research Use Only
  • Concentration
    0.1mg/ml
  • Scientific Description
    The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex witHuman, MouseCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by, protein kinases CDC2 and CDC7.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MCM2 using anti-MCM2 antibody (A00374-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human THP-1 whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: rat ovarian tissue lysates, Lane 6: mouse ovarian tissue lysates, Lane 7: mouse NIH3T3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MCM2 antigen affinity purified polyclonal antibody (Catalog # A00374-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MCM2 at approximately 125KD. The expected band size for MCM2 is at 102KD.
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