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Western Blot analysis of HepG2 cells using CDC46 Polyclonal Antibody
Western Blot analysis of HepG2 cells using CDC46 Polyclonal Antibody
Western Blot analysis of HepG2 cells using CDC46 Polyclonal Antibody

MCM5 Antibody

CSB-PA001513
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetMCM5
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Overview

  • Supplier
    Cusabio
  • Product Name
    MCM5 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4174
  • Target name
    MCM5
  • Target description
    minichromosome maintenance complex component 5
  • Target synonyms
    CDC46, MGORS8, P1-CDC46, DNA replication licensing factor MCM5, CDC46 homolog, MCM5 minichromosome maintenance deficient 5, cell division cycle 46, minichromosome maintenance deficient 5 (cell division cycle 46)
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP33992
  • Protein Name
    DNA replication licensing factor MCM5
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of MCM5 using anti-MCM5 antibody (A03642). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: rat thymus tissue lysates, Lane 3: mouse thymus tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MCM5 antigen affinity purified polyclonal antibody (Catalog # A03642) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MCM5 at approximately 100KD. The expected band size for MCM5 is at 82KD.
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