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Formalin-fixed and paraffin embedded human gastric carcinoma labeled with Anti-MCM5 Polyclonal Antibody, Unconjugated (bs-2721R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\n
Formalin-fixed and paraffin embedded human gastric carcinoma labeled with Anti-MCM5 Polyclonal Antibody, Unconjugated (bs-2721R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\n
Formalin-fixed and paraffin embedded human gastric carcinoma labeled with Anti-MCM5 Polyclonal Antibody, Unconjugated (bs-2721R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\n

MCM5 Polyclonal Antibody

BS-2721R
Bioss
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetMCM5
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Overview

  • Supplier
    Bioss
  • Product Name
    MCM5 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID4174
  • Target name
    MCM5
  • Target description
    minichromosome maintenance complex component 5
  • Target synonyms
    CDC46, MGORS8, P1-CDC46, DNA replication licensing factor MCM5, CDC46 homolog, MCM5 minichromosome maintenance deficient 5, cell division cycle 46, minichromosome maintenance deficient 5 (cell division cycle 46)
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP33992
  • Protein Name
    DNA replication licensing factor MCM5
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

Datasheet

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Figure 1. Western blot analysis of MCM5 using anti-MCM5 antibody (A03642). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: rat thymus tissue lysates, Lane 3: mouse thymus tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MCM5 antigen affinity purified polyclonal antibody (Catalog # A03642) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MCM5 at approximately 100KD. The expected band size for MCM5 is at 82KD.
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