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Western Blot analysis of 293 cells using MCM7 Polyclonal Antibody.
Western Blot analysis of 293 cells using MCM7 Polyclonal Antibody.
Western Blot analysis of 293 cells using MCM7 Polyclonal Antibody.

MCM7 Antibody

CSB-PA005604
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetMCM7
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Overview

  • Supplier
    Cusabio
  • Product Name
    MCM7 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4176
  • Target name
    MCM7
  • Target description
    minichromosome maintenance complex component 7
  • Target synonyms
    CDC47; CDC47 homolog; DNA replication licensing factor MCM7; homolog of S. cerevisiae Cdc47; MCM2; minichromosome maintenance deficient 7; P1.1-MCM3; P1CDC47; P85MCM; PNAS146; PPP1R104; protein phosphatase 1, regulatory subunit 104
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP33993
  • Protein Name
    DNA replication licensing factor MCM7
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of MCM7 using anti-MCM7 antibody (PB9261). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MCM7 antigen affinity purified polyclonal antibody (Catalog # PB9261) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MCM7 at approximately 81 kDa. The expected band size for MCM7 is at 81 kDa.
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