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ProductsBack/MCM7 antibody [N2C2], Internal
Immunohistochemical analysis of paraffin-embedded Cal27 Xenograft, using MCM7(GTX110278) antibody at 1:100 dilution. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
Immunohistochemical analysis of paraffin-embedded Cal27 Xenograft, using MCM7(GTX110278) antibody at 1:100 dilution. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
Immunohistochemical analysis of paraffin-embedded Cal27 Xenograft, using MCM7(GTX110278) antibody at 1:100 dilution. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min

MCM7 antibody [N2C2], Internal

GTX110278
GeneTex
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetMCM7
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Overview

  • Supplier
    GeneTex
  • Product Name
    MCM7 antibody [N2C2], Internal
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.76 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID4176
  • Target name
    MCM7
  • Target description
    minichromosome maintenance complex component 7
  • Target synonyms
    CDC47, MCM2, P1.1-MCM3, P1CDC47, P85MCM, PNAS146, PPP1R104, DNA replication licensing factor MCM7, CDC47 homolog, homolog of S. cerevisiae Cdc47, minichromosome maintenance deficient 7, protein phosphatase 1, regulatory subunit 104
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP33993
  • Protein Name
    DNA replication licensing factor MCM7
  • Scientific Description
    The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 6 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. Cyclin D1-dependent kinase, CDK4, is found to associate with this protein, and may regulate the binding of this protein with the tumorsuppressor protein RB1/RB. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq]
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Sikorski K, Mehta A, Inngjerdingen M, et al. A high-throughput pipeline for validation of antibodies. Nat Methods. 2018,15(11):909-912. doi: 10.1038/s41592-018-0179-8
    Read this paper

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