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Western blot All lanes: MCM9 antibody at 0.8ug/ml Lane 1: Mouse brain tissue Lane 2: 293T whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 128, 73, 44, 85 kDa Observed band size: 128 kDa
Western blot All lanes: MCM9 antibody at 0.8ug/ml Lane 1: Mouse brain tissue Lane 2: 293T whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 128, 73, 44, 85 kDa Observed band size: 128 kDa
Western blot All lanes: MCM9 antibody at 0.8ug/ml Lane 1: Mouse brain tissue Lane 2: 293T whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 128, 73, 44, 85 kDa Observed band size: 128 kDa

MCM9 Antibody

CSB-PA882135LA01HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetMCM9
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Overview

  • Supplier
    Cusabio
  • Product Name
    MCM9 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID254394
  • Target name
    MCM9
  • Target description
    minichromosome maintenance 9 homologous recombination repair factor
  • Target synonyms
    C6orf61, MCMDC1, ODG4, dJ329L24.1, dJ329L24.3, DNA helicase MCM9, DNA replication licensing factor MCM9, mini-chromosome maintenance deficient domain-containing protein 1, minichromosome maintenance complex component 9
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NXL9
  • Protein Name
    DNA helicase MCM9
  • Scientific Description
    Component of the MCM8-MCM9 complex, a complex involved in homologous recombination repair following DNA interstrand cross-links and plays a key role during gametogenesis. The MCM8-MCM9 complex probably acts as a hexameric helicase downstream of the Fanconi anemia proteins BRCA2 and RAD51 and is required to process aberrant forks into homologous recombination substrates and to orchestrate homologous recombination with resection, fork stabilization and fork restart.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of MCM9 using anti-MCM9 antibody (A07226-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MCM9 antigen affinity purified polyclonal antibody (Catalog # A07226-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MCM9 at approximately 127 kDa. The expected band size for MCM9 is at 127 kDa.
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