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WB analysis of Jurkat cells using GTX47470 MCM9 antibody at 0.2-1microg/ml.
WB analysis of Jurkat cells using GTX47470 MCM9 antibody at 0.2-1microg/ml.
WB analysis of Jurkat cells using GTX47470 MCM9 antibody at 0.2-1microg/ml.

MCM9 antibody, N-term

GTX47470
GeneTex
ApplicationsWestern Blot
Product group Antibodies
TargetMCM9
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Overview

  • Supplier
    GeneTex
  • Product Name
    MCM9 antibody, N-term
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 0.2-2.5 ug/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5-1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID254394
  • Target name
    MCM9
  • Target description
    minichromosome maintenance 9 homologous recombination repair factor
  • Target synonyms
    C6orf61, MCMDC1, ODG4, dJ329L24.1, dJ329L24.3, DNA helicase MCM9, DNA replication licensing factor MCM9, mini-chromosome maintenance deficient domain-containing protein 1, minichromosome maintenance complex component 9
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NXL9
  • Protein Name
    DNA helicase MCM9
  • Scientific Description
    The protein encoded by this gene is a member of the mini-chromosome maintenance (MCM) protein family that are essential for the initiation of eukaryotic genome replication. Binding of this protein to chromatin has been shown to be a pre-requisite for recruiting the MCM2-7 helicase to DNA replication origins. This protein also binds, and is a positive regulator of, the chromatin licensing and DNA replication factor 1, CDT1. [provided by RefSeq, Nov 2010]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MCM9 using anti-MCM9 antibody (A07226-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MCM9 antigen affinity purified polyclonal antibody (Catalog # A07226-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MCM9 at approximately 127 kDa. The expected band size for MCM9 is at 127 kDa.
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