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ProductsBack/MCP1 / CCL2 antibody
WB analysis of LPS stimulated RAW cells (1) and rMCP-1 transfected cells (2) using GTX27202 MCP1 / CCL2 antibody.
WB analysis of LPS stimulated RAW cells (1) and rMCP-1 transfected cells (2) using GTX27202 MCP1 / CCL2 antibody.
WB analysis of LPS stimulated RAW cells (1) and rMCP-1 transfected cells (2) using GTX27202 MCP1 / CCL2 antibody.

MCP1 / CCL2 antibody

GTX27202
GeneTex
ApplicationsWestern Blot
Product group Antibodies
ReactivityMouse, Rat
TargetCcl2
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Overview

  • Supplier
    GeneTex
  • Product Name
    MCP1 / CCL2 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: Use at a dilution of 1:2,000. Optimal dilutions/concentrations should be determined by the end user.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID24770
  • Target name
    Ccl2
  • Target description
    C-C motif chemokine ligand 2
  • Target synonyms
    MCP-1, Scya2, Sigje, C-C motif chemokine 2, chemokine (C-C motif) ligand 2, immediate-early serum-responsive JE protein, immediate-early serum-responsive protein JE, monocyte chemoattractant protein 1, monocyte chemotactic protein 1, small inducible cytokine A2, small inducible gene JE
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP14844
  • Protein Name
    C-C motif chemokine 2
  • Scientific Description
    a monocyte chemoattractant protein [RGD, Feb 2006]
  • Reactivity
    Mouse, Rat
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CCL2 using anti-CCL2 antibody (A00056-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat pancreas tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCL2 antigen affinity purified polyclonal antibody (Catalog # A00056-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CCL2 at approximately 11KD. The expected band size for CCL2 is at 11KD.
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