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MCP1 Polyclonal Antibody

BS-1101R
Bioss
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetCcl2
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Overview

  • Supplier
    Bioss
  • Product Name
    MCP1 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID24770
  • Target name
    Ccl2
  • Target description
    C-C motif chemokine ligand 2
  • Target synonyms
    MCP-1, Scya2, Sigje, C-C motif chemokine 2, chemokine (C-C motif) ligand 2, immediate-early serum-responsive JE protein, immediate-early serum-responsive protein JE, monocyte chemoattractant protein 1, monocyte chemotactic protein 1, small inducible cytokine A2, small inducible gene JE
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP14844
  • Protein Name
    C-C motif chemokine 2
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Zheng HX, Qi SS, He J, et al. Cyanidin-3-glucoside from Black Rice Ameliorates Diabetic Nephropathy via Reducing Blood Glucose, Suppressing Oxidative Stress and Inflammation, and Regulating Transforming Growth Factor β1/Smad Expression. J Agric Food Chem. 2020,68(15):4399-4410. doi: 10.1021/acs.jafc.0c00680
    Read this paper
  • Gao X, Zhu R, Du J, et al. Inhibition of LOX-1 prevents inflammation and photoreceptor cell death in retinal degeneration. Int Immunopharmacol. 2020,80:106190. doi: 10.1016/j.intimp.2020.106190
    Read this paper
  • Zhang P, Hua L, Hou H, et al. Sphingomyelin synthase 2 promotes H2O2-induced endothelial dysfunction by activating the Wnt/β-catenin signaling pathway. Int J Mol Med. 2018,42(6):3344-3354. doi: 10.3892/ijmm.2018.3888
    Read this paper
  • Li X, Fang Q, Tian X, et al. Curcumin attenuates the development of thoracic aortic aneurysm by inhibiting VEGF expression and inflammation. Mol Med Rep. 2017,16(4):4455-4462. doi: 10.3892/mmr.2017.7169
    Read this paper
  • He Y, Xu Z, Fu H, et al. Combined Microencapsulated Islet Transplantation and Revascularization of Aortorenal Bypass in a Diabetic Nephropathy Rat Model. J Diabetes Res. 2016,2016:9706321. doi: 10.1155/2016/9706321
    Read this paper
  • Iwasa A, Arakaki R, Honma N, et al. Aromatase controls Sjögren syndrome-like lesions through monocyte chemotactic protein-1 in target organ and adipose tissue-associated macrophages. Am J Pathol. 2015,185(1):151-61. doi: 10.1016/j.ajpath.2014.09.006
    Read this paper
  • Su X, Zhou G, Wang Y, et al. The PPARβ/δ agonist GW501516 attenuates peritonitis in peritoneal fibrosis via inhibition of TAK1-NFκB pathway in rats. Inflammation. 2014,37(3):729-37. doi: 10.1007/s10753-013-9791-z
    Read this paper
  • Guo Z, Zhang R, Li J, et al. Effect of telmisartan on the expression of adiponectin receptors and nicotinamide adenine dinucleotide phosphate oxidase in the heart and aorta in type 2 diabetic rats. Cardiovasc Diabetol. 2012,11:94. doi: 10.1186/1475-2840-11-94
    Read this paper

Datasheet

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Figure 1. Western blot analysis of CCL2 using anti-CCL2 antibody (A00056-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat pancreas tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCL2 antigen affinity purified polyclonal antibody (Catalog # A00056-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CCL2 at approximately 11KD. The expected band size for CCL2 is at 11KD.
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