MCP1 / CCL2 antibody [S14]

GeneTex

MCP1 / CCL2 antibody [S14]

9

This monoclonal antibody reacts with natural and recombinant human MCP-1. Does not react with human interleukin-8 (IL-8) and other human cytokines tested such as interleukin-1? (IL-1?), serum amyloid A (SAA) and epidermal growth factor (EGF).

For ELISA: Use at an assay dependent dilution. This antibody can be used as a capture antibody in sandwich ELISA applications for human MCP-1 detection in combination with a monoclonal tracer/detection antibody (HRP-conjugated or biotin-conjugated). Suggested Capture Coating Dose: 0.3microg/well; Substrate: TMB. If the above suggested conditions are followed approximately 1.5 pg/mL of MCP-1 in serum/plasma or 4 pg/mL of MCP-1 in medium can be detected with an assay range of 0-1600 pg/mL. For Neut: Use at a concentration of 0.5 microg/ml. Tests on the ability to inhibit monocyte chemotaxis toward 1 nM recombinant human MCP-1 in blindwell chambers showed that the antibodies were particularly effective at blocking MCP-1 activity at a concentration of 0.5microg/mL. It was also found that S-14 could inhibit the function of native MCP-1 at concentrations similar to inhibitory doses for recombinant MCP-1. For WB: Use at a concentration of 0.1-1.0 microg/ml. Optimal dilutions/concentrations should be determined by the researcher.

Western Blot, ELISA, Neutralisation/Blocking

Research Use Only

Monoclonal

S14

1 mg/ml

Unconjugated

Liquid

CCL2

C-C motif chemokine ligand 2

C-C motif chemokine 2; chemokine (C-C motif) ligand 2; GDCF-2; HC11; HSMCR30; MCAF; MCP1; MCP-1; monocyte chemoattractant protein-1; monocyte chemotactic and activating factor; monocyte chemotactic protein 1; monocyte secretory protein JE; SCYA2; small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig-je); small inducible cytokine subfamily A (Cys-Cys), member 2; small-inducible cytokine A2; SMC-CF

Mouse

IgG1

C-C motif chemokine 2

This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of N-terminal cysteine residues of the mature peptide. This chemokine is a member of the CC subfamily which is characterized by two adjacent cysteine residues. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4. [provided by RefSeq, Jul 2013]

Human

-20°C or -80°C,2°C to 8°C

12352203