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MDH1 antibody

70R-18451
Biosynth
Product group Antibodies
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Overview

  • Supplier
    Biosynth
  • Product Name
    MDH1 antibody
  • Delivery Days Customer
    11
  • Certification
    Research Use Only
  • UNSPSC
    12352203

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RayBiotech
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TargetMDH1
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Figure 1. Western blot analysis of MDH1 using anti-MDH1 antibody (A04262-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human HL-60 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat spleen tissue lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse spleen tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MDH1 antigen affinity purified polyclonal antibody (Catalog # A04262-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MDH1 at approximately 36 kDa. The expected band size for MDH1 is at 59 kDa.
Product group Antibodies
Boster Bio
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TargetMDH1
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Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with MDH1 antibody (GTX130900) diluted at 1:1000.
Product group Antibodies
References
GeneTex
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Lane 1: MOLT-4 cell lysates; Lane 2: Jurkat cell lysates probed with MDH1 Polyclonal Antibody, Unconjugated (bs-3996R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Product group Antibodies
References
Bioss
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TargetMDH1
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Western Blot Positive WB detected in: HL60 whole cell lysate, HepG2 whole cell lysate, Hela whole cell lysate, 293 whole cell lysate, Jurkat whole cell lysate, LO2 whole cell lysate, NIH/3T3 whole cell lysate, RAW264.7 whole cell lysate, Mouse liver tissue All lanes: MDH1 antibody at 2.6microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 37, 28, 39 kDa Observed band size: 37 kDa
Product group Antibodies
Cusabio
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ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse
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Immunohistochemical staining of human kidney shows moderate cytoplasmic positivity in cells in tubules.
Product group Antibodies
Atlas Antibodies
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