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ProductsBack/Goat anti-MDH1 / MOR2

Goat anti-MDH1 / MOR2

EB11994
Everest Biotech
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetMDH1
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-MDH1 / MOR2 Antibody
  • Delivery Days Customer
    5
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    Pep-ELISA, WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID4190
  • Target name
    MDH1
  • Target description
    malate dehydrogenase 1
  • Target synonyms
    cytosolic malate dehydrogenase; DEE88; diiodophenylpyruvate reductase; EIEE88; epididymis secretory protein Li 32; HEL-S-32; KAR; malate dehydrogenase 1, NAD (soluble); malate dehydrogenase, cytoplasmic; malate dehydrogenase, peroxisomal; MDHA; MDH-s; MGC:1375; MOR2; soluble malate dehydrogenase
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_001186040.1; NP_005908.1; NP_001186041.1. GeneIDs all Nonhuman: 17449 (mouse); 24551 (rat);. Purification: Antigen affinity purified. Names and symbols: MDH1; malate dehydrogenase 1, NAD (soluble); MDH-s; MDHA; MGC:1375; MOR2; cytosolic malate dehydrogenase; malate dehydrogenase, cytoplasmic; soluble malate dehydrogenase
  • Reactivity Supplier
    Human, Mouse, Rat, Dog, Pig, Cow
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MDH1 using anti-MDH1 antibody (A04262-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human HL-60 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat spleen tissue lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse spleen tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MDH1 antigen affinity purified polyclonal antibody (Catalog # A04262-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MDH1 at approximately 36 kDa. The expected band size for MDH1 is at 59 kDa.
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Lane 1: MOLT-4 cell lysates; Lane 2: Jurkat cell lysates probed with MDH1 Polyclonal Antibody, Unconjugated (bs-3996R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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