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Immunohistochemistry of paraffin-embedded Human colon cancer tissue using MDK Polyclonal Antibody at dilution 1:60
Immunohistochemistry of paraffin-embedded Human colon cancer tissue using MDK Polyclonal Antibody at dilution 1:60
Immunohistochemistry of paraffin-embedded Human colon cancer tissue using MDK Polyclonal Antibody at dilution 1:60

MDK Polyclonal Antibody

E-AB-10458
Elabscience
ApplicationsImmunoHistoChemistry
Product group Antibodies
TargetMDK
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Overview

  • Supplier
    Elabscience
  • Product Name
    MDK Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    ImmunoHistoChemistry
  • Applications Supplier
    ELISA IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.2 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID4192
  • Target name
    MDK
  • Target description
    midkine
  • Target synonyms
    ARAP, MK, NEGF2, midkine, amphiregulin-associated protein, midgestation and kidney protein, neurite growth-promoting factor 2, neurite outgrowth-promoting factor 2, retinoic acid inducible factor
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP21741
  • Protein Name
    Midkine
  • Scientific Description
    This gene encodes a member of a small family of secreted growth factors that binds heparin and responds to retinoic acid. The encoded protein promotes cell growth, migration, and angiogenesis, in particular during tumorigenesis. This gene has been targeted as a therapeutic for a variety of different disorders. Alternatively spliced transcript variants encoding multiple isoforms have been observed.
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of MDK using anti-MDK antibody (RP1051). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human U20S whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MDK antigen affinity purified polyclonal antibody (Catalog # RP1051) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MDK at approximately 16 kDa. The expected band size for MDK is at 16 kDa.
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