More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/MDM2 Rabbit pAb

MDM2 Rabbit pAb

A0345
ABclonal
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetMDM2
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    ABclonal
  • Product Name
    MDM2 Rabbit pAb
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4193
  • Target name
    MDM2
  • Target description
    MDM2 proto-oncogene
  • Target synonyms
    ACTFS, HDMX, LSKB, hdm2, E3 ubiquitin-protein ligase Mdm2, MDM2 oncogene, E3 ubiquitin protein ligase, MDM2 proto-oncogene, E3 ubiquitin protein ligase, Mdm2, p53 E3 ubiquitin protein ligase homolog, Mdm2, transformed 3T3 cell double minute 2, p53 binding protein, double minute 2, human homolog of; p53-binding protein, oncoprotein Mdm2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ00987
  • Protein Name
    E3 ubiquitin-protein ligase Mdm2
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

FAQ

Go to our frequently asked questions

Related products

Product group Antibodies
Assay Genie
MDM2 AntibodyCAB0345
ReactivityHuman
TargetMDM2
  • SizePrice
Western Blot analysis of Hela cells using MDM2 Polyclonal Antibody
Product group Antibodies
Cusabio
MDM2 AntibodyCSB-PA003212
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Monkey, Mouse
TargetMDM2
  • SizePrice
Figure 1. Western blot analysis of MDM2 using anti-MDM2 antibody (A00054-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates, Lane 2: rat PC-12 whole cell lysates, Lane 3: rat RH-35 whole cell lysates, Lane 4: mouse HEPA1-6 whole cell lysates, Lane 5: mouse NTH/3T3 whole cell lysates, Lane 6: mouse ANA-1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MDM2 antigen affinity purified polyclonal antibody (Catalog # A00054-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MDM2 at approximately 90KD. The expected band size for MDM2 is at 90KD.
Product group Antibodies
Boster Bio
Anti-MDM2 Antibody Picoband(r)A00054-2-CARRIER-FREE
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetMDM2
  • SizePrice
Anti-MDM2 Antibody
Product group Antibodies
Antibodies.com
Anti-MDM2 AntibodyA101381
ApplicationsWestern Blot, ELISA
ReactivityHuman
  • SizePrice
Product group Antibodies
RayBiotech
Anti-MDM2 Antibody144-60282
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetMDM2
  • SizePrice
Product group Antibodies
MedChem Express
Phospho-MDM2 (Ser166) Antibody (YA1888, Rabbit)HY-P82143
TargetMDM2
  • SizePrice
Product group Antibodies
Everest Biotech
Goat anti-MDM2 (isoform)EB06990
ApplicationsWestern Blot, ELISA
ReactivityBovine, Canine, Feline, Human, Porcine, Rat
TargetMDM2
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
MDM2 Antibody (C-Terminus)LS-C368433
ApplicationsWestern Blot
ReactivityBovine, Canine, Human, Mouse, Rat
TargetMDM2
  • SizePrice
Product group Antibodies
Biomatik
MDM2 Polyclonal AntibodyCAC15788
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
TargetMDM2
  • SizePrice
Paraformaldehyde-fixed, paraffin embedded human colon carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with phospho-MDM2 (Ser166) Polyclonal Antibody, Unconjugated (bs-3266R) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
Product group Antibodies
Bioss
Phospho-MDM2 (Ser166) Polyclonal AntibodyBS-3266R
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityEquine, Human, Mouse, Rabbit, Rat
TargetMDM2
  • SizePrice