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Western Blot analysis of RAW264.7 cells using ME3 Polyclonal Antibody
Western Blot analysis of RAW264.7 cells using ME3 Polyclonal Antibody
Western Blot analysis of RAW264.7 cells using ME3 Polyclonal Antibody

ME3 Antibody

CSB-PA006739
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetME3
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Overview

  • Supplier
    Cusabio
  • Product Name
    ME3 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10873
  • Target name
    ME3
  • Target description
    malic enzyme 3
  • Target synonyms
    malate dehydrogenase (oxaloacetate-decarboxylating) (NADP(+)); malic enzyme 3, NADP(+)-dependent, mitochondrial; malic enzyme, NADP+-dependent, mitochondrial; mitochondrial NADP(+)-dependent malic enzyme 3; NADP-dependent malic enzyme, mitochondrial; NADP-ME; pyruvic-malic carboxylase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ16798
  • Protein Name
    NADP-dependent malic enzyme, mitochondrial
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of ME3 using anti-ME3 antibody (A05164-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: monkey COS7 whole cell lysates, Lane 2: human HEL whole cell lysates, Lane 3: rat kidney tissue lysates, Lane 4: rat heart tissue lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse kidney tissue lysates, Lane 8: mouse heart tissue lysates, Lane 9: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ME3 antigen affinity purified polyclonal antibody (Catalog # A05164-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ME3 at approximately 67 kDa. The expected band size for ME3 is at 67 kDa.
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