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ME3 Polyclonal Antibody

CAC14561
Biomatik
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetME3
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Overview

  • Supplier
    Biomatik
  • Product Name
    ME3 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10873
  • Target name
    ME3
  • Target description
    malic enzyme 3
  • Target synonyms
    malate dehydrogenase (oxaloacetate-decarboxylating) (NADP(+)); malic enzyme 3, NADP(+)-dependent, mitochondrial; malic enzyme, NADP+-dependent, mitochondrial; mitochondrial NADP(+)-dependent malic enzyme 3; NADP-dependent malic enzyme, mitochondrial; NADP-ME; pyruvic-malic carboxylase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ16798
  • Protein Name
    NADP-dependent malic enzyme, mitochondrial
  • Scientific Description
    The ME3 Polyclonal Antibody (Species: Human) has been validated for the following applications: ELISA, WB, IHC.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ME3 using anti-ME3 antibody (A05164-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: monkey COS7 whole cell lysates, Lane 2: human HEL whole cell lysates, Lane 3: rat kidney tissue lysates, Lane 4: rat heart tissue lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse kidney tissue lysates, Lane 8: mouse heart tissue lysates, Lane 9: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ME3 antigen affinity purified polyclonal antibody (Catalog # A05164-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ME3 at approximately 67 kDa. The expected band size for ME3 is at 67 kDa.
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