ProductsBack/MECP2 antibody - ChIP grade

ChIP analysis of sheared chromatin from 10? U2OS cells using GTX60358 MECP2 antibody - ChIP grade. IgG (1 microg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the ZMYND10 gene (used as a positive control) and CDC6 gene (used as a negative control). This figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

MECP2 antibody - ChIP grade

GTX60358

ApplicationsWestern Blot, ChIP Chromatin ImmunoPrecipitation, ELISA

DownloadDatasheet

Product group Antibodies

TargetMECP2

Overview

Supplier
GeneTex
Product Name
MECP2 antibody - ChIP grade
Delivery Days Customer
9
Application Supplier Note
WB: 1:1,000. ELISA: 1:1,000. ChIP assay: 1-5 microg. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Applications
Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA
Certification
Research Use Only
Clonality
Polyclonal
Concentration
1.2 mg/ml
Conjugate
Unconjugated
Gene ID4204
Target name
MECP2
Target description
methyl-CpG binding protein 2
Target synonyms
AUTSX3; meCp-2 protein; methyl-CpG-binding protein 2; MRX16; MRX79; MRXS13; MRXSL; PPMX; RS; RTS; RTT
Host
Rabbit
Isotype
IgG
Protein IDP51608
Protein Name
Methyl-CpG-binding protein 2
Scientific Description
DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. In contrast to other MBD family members, MECP2 is X-linked and subject to X inactivation. MECP2 is dispensible in stem cells, but is essential for embryonic development. MECP2 gene mutations are the cause of most cases of Rett syndrome, a progressive neurologic developmental disorder and one of the most common causes of cognitive disability in females. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2015]
Storage Instruction
-20°C or -80°C,2°C to 8°C
UNSPSC
12352203