ChIP analysis of sheared chromatin from 10? U2OS cells using GTX60358 MECP2 antibody - ChIP grade. IgG (1 microg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the ZMYND10 gene (used as a positive control) and CDC6 gene (used as a negative control). This figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
ChIP analysis of sheared chromatin from 10? U2OS cells using GTX60358 MECP2 antibody - ChIP grade. IgG (1 microg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the ZMYND10 gene (used as a positive control) and CDC6 gene (used as a negative control). This figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
MECP2 antibody - ChIP grade
GTX60358
ApplicationsWestern Blot, ChIP Chromatin ImmunoPrecipitation, ELISA
Product group Antibodies
ReactivityHuman
TargetMECP2
Overview
- SupplierGeneTex
- Product NameMECP2 antibody - ChIP grade
- Delivery Days Customer9
- Application Supplier NoteWB: 1:1,000. ELISA: 1:1,000. ChIP assay: 1-5 microg. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot, ChIP Chromatin ImmunoPrecipitation, ELISA
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1.2 mg/ml
- ConjugateUnconjugated
- Gene ID4204
- Target nameMECP2
- Target descriptionmethyl-CpG binding protein 2
- Target synonymsAUTSX3; meCp-2 protein; methyl-CpG-binding protein 2; MRX16; MRX79; MRXS13; MRXSL; PPMX; RS; RTS; RTT
- HostRabbit
- IsotypeIgG
- Protein IDP51608
- Protein NameMethyl-CpG-binding protein 2
- Scientific DescriptionDNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. In contrast to other MBD family members, MECP2 is X-linked and subject to X inactivation. MECP2 is dispensible in stem cells, but is essential for embryonic development. MECP2 gene mutations are the cause of most cases of Rett syndrome, a progressive neurologic developmental disorder and one of the most common causes of cognitive disability in females. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2015]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Multiple epigenetic biomarkers for evaluation of students academic performance. Lee LC et al., 2019 Jun, Genes Brain BehavRead more