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Immunohistochemistry of paraffin-embedded human tonsil tissue using CSB-PA013638ESR2HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human tonsil tissue using CSB-PA013638ESR2HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human tonsil tissue using CSB-PA013638ESR2HU at dilution of 1:100

MECP2 Antibody

CSB-PA013638ESR2HU
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetMECP2
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Overview

  • Supplier
    Cusabio
  • Product Name
    MECP2 Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4204
  • Target name
    MECP2
  • Target description
    methyl-CpG binding protein 2
  • Target synonyms
    AUTSX3, MRX16, MRX79, MRXS13, MRXSL, PPMX, RS, RTS, RTT, methyl-CpG-binding protein 2, meCp-2 protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP51608
  • Protein Name
    Methyl-CpG-binding protein 2
  • Scientific Description
    Chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair. It is not influenced by sequences flanking the methyl-CpGs. Mediates transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A. Binds both 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC)-containing DNA, with a preference for 5-methylcytosine (5mC) (By similarity).
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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ChIP analysis of sheared chromatin from 10? U2OS cells using GTX60358 MECP2 antibody - ChIP grade. IgG (1 microg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the ZMYND10 gene (used as a positive control) and CDC6 gene (used as a negative control). This figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
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Figure 1. Western blot analysis of MECP2 using anti-MECP2 antibody (A00047-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MDA-MB-453 whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: monkey COS-7 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MECP2 antigen affinity purified polyclonal antibody (Catalog # A00047-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MECP2 at approximately 80 kDa. The expected band size for MECP2 is at 52 kDa.
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Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with MeCP2 Polyclonal Antibody, Unconjugated (bs-22098R) at 1:200 overnight at 4°C, DAB staining.
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Immunohistochemical staining of human cerebellum, lymph node, prostate and testis using Anti-MECP2 antibody HPA000593 (A) shows similar protein distribution across tissues to independent antibody HPA001341 (B).
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