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MED14 Antibody

ORB193494
Biorbyt
ApplicationsImmunoFluorescence, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetMED14
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Overview

  • Supplier
    Biorbyt
  • Product Name
    MED14 Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, ImmunoHistoChemistry
  • Applications Supplier
    IF, IHC
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Gene ID9282
  • Target name
    MED14
  • Target description
    mediator complex subunit 14
  • Target synonyms
    CRSP150, CRSP2, CSRP, CXorf4, DRIP150, EXLM1, RGR1, TRAP170, mediator of RNA polymerase II transcription subunit 14, ARC150, CRSP complex subunit 2, RGR1 homolog, activator-recruited cofactor 150 kDa component, cofactor required for Sp1 transcriptional activation, subunit 2 (150kD), hRGR1, human homolog of yeast RGR1, thyroid hormone receptor-associated protein complex 170 kDa component, thyroid hormone receptor-associated protein complex component TRAP170, transcriptional co-activator CRSP150, vitamin D receptor-interacting protein complex component DRIP150, vitamin D3 receptor-interacting protein complex 150 kDa component
  • Host
    Rabbit
  • Protein IDO60244
  • Protein Name
    Mediator of RNA polymerase II transcription subunit 14
  • Scientific Description
    MED14 Antibody
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MED14 using anti-MED14 antibody (A04799-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: mouse liver tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MED14 antigen affinity purified polyclonal antibody (Catalog # A04799-2) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MED14 at approximately 160KD. The expected band size for MED14 is at 160KD.
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