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ProductsBack/MED17 / TRAP80 Antibody

MED17 / TRAP80 Antibody

LS-C410254
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetMED17
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    MED17 / TRAP80 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 15kDa/72kDa, while the observed MW by Western blot was 80kDa.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:200 - 1:2000) The predicted MW is 15kDa/72kDa, while the observed MW by Western blot was 80kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID9440
  • Target name
    MED17
  • Target description
    mediator complex subunit 17
  • Target synonyms
    CRSP6, CRSP77, DRIP80, SRB4, TRAP80, mediator of RNA polymerase II transcription subunit 17, ARC77, CRSP complex subunit 6, activator-recruited cofactor 77 kDa component, cofactor required for Sp1 transcriptional activation, subunit 6, 77kDa, epididymis secretory sperm binding protein, thyroid hormone receptor-associated protein complex 80 kDa component, transcriptional coactivator CRSP77, vitamin D3 receptor-interacting protein complex 80 kDa component
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MED17 using anti-MED17 antibody (A05399-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: human SIHA whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MED17 antigen affinity purified polyclonal antibody (Catalog # A05399-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MED17 at approximately 73 kDa. The expected band size for MED17 is at 73 kDa.
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