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Western blot All lanes: MED6 antibody at 8ug/ml + Jurkat whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 29, 30, 21 kDa Observed band size: 29 kDa
Western blot All lanes: MED6 antibody at 8ug/ml + Jurkat whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 29, 30, 21 kDa Observed band size: 29 kDa
Western blot All lanes: MED6 antibody at 8ug/ml + Jurkat whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 29, 30, 21 kDa Observed band size: 29 kDa

MED6 Antibody

CSB-PA013666ESR1HU
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetMED6
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Overview

  • Supplier
    Cusabio
  • Product Name
    MED6 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10001
  • Target name
    MED6
  • Target description
    mediator complex subunit 6
  • Target synonyms
    activator-recruited cofactor 33 kDa component; ARC33; CTD-2540L5.5; mediator of RNA polymerase II transcription subunit 6; NY-REN-28; renal carcinoma antigen NY-REN-28
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO75586
  • Protein Name
    Mediator of RNA polymerase II transcription subunit 6
  • Scientific Description
    Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of MED6 using anti-MED6 antibody (A09224). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human PC-3 whole cell lysates, Lane 5: human U2OS whole cell lysates, Lane 6: human A549 whole cell lysates, Lane 7: human MCF-7 whole cell lysates, Lane 8: rat C6 whole cell lysates. Lane 9: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MED6 antigen affinity purified polyclonal antibody (Catalog # A09224) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MED6 at approximately 34 kDa. The expected band size for MED6 is at 28 kDa.
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