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Anti-MED6 Antibody

CAB8181
Assay Genie
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetMED6
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Overview

  • Supplier
    Assay Genie
  • Product Name
    Anti-MED6 Antibody
  • Delivery Days Customer
    9
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Clone ID
    Not applicable
  • Conjugate
    Unconjugated
  • Gene ID10001
  • Target name
    MED6
  • Target description
    mediator complex subunit 6
  • Target synonyms
    ARC33, NY-REN-28, mediator of RNA polymerase II transcription subunit 6, CTD-2540L5.5, activator-recruited cofactor 33 kDa component, renal carcinoma antigen NY-REN-28
  • Host
    Rabbit
  • Protein IDO75586
  • Protein Name
    Mediator of RNA polymerase II transcription subunit 6
  • Scientific Description
    MED6 Antibody is a premium polyclonal that offers outstanding performance and reliability for demanding research applications. Rigorously validated for WB, ELISA, this antibody ensures consistent, reproducible results across multiple experimental platforms. Demonstrates excellent reactivity with Human samples, providing researchers with confidence in cross-species compatibility. Conveniently packaged in 100microL format to meet your experimental needs. For optimal performance, store at -20°C and maintains stability for 12 months. Backed by rigorous quality control testing to ensure superior performance in your critical research applications.
  • Shelf life instruction
    12 months
  • Source
    Rabbit
  • Reactivity
    Human
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of MED6 using anti-MED6 antibody (A09224). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human PC-3 whole cell lysates, Lane 5: human U2OS whole cell lysates, Lane 6: human A549 whole cell lysates, Lane 7: human MCF-7 whole cell lysates, Lane 8: rat C6 whole cell lysates. Lane 9: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MED6 antigen affinity purified polyclonal antibody (Catalog # A09224) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MED6 at approximately 34 kDa. The expected band size for MED6 is at 28 kDa.
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