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Western blot analysis of extracts from COLO cells, using MEGF9 antibody.The lane on the right is treated with the synthesized peptide.
Western blot analysis of extracts from COLO cells, using MEGF9 antibody.The lane on the right is treated with the synthesized peptide.
Western blot analysis of extracts from COLO cells, using MEGF9 antibody.The lane on the right is treated with the synthesized peptide.

MEGF9 Rabbit Polyclonal Antibody

TA313794
OriGene
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse
TargetMEGF9
100 ul
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Overview

  • Supplier
    OriGene
  • Product Name
    MEGF9 Rabbit Polyclonal Antibody
  • Delivery Days Customer
    14
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID1955
  • Target name
    MEGF9
  • Target description
    multiple EGF like domains 9
  • Target synonyms
    EGFL5, multiple epidermal growth factor-like domains protein 9, EGF-like-domain, multiple 5, epidermal growth factor-like protein 5
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9H1U4
  • Protein Name
    Multiple epidermal growth factor-like domains protein 9
  • Scientific Description
    Rabbit polyclonal anti-MEGF9 antibody
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of MEGF9 using anti-MEGF9 antibody (A15544-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human RT4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MEGF9 antigen affinity purified polyclonal antibody (Catalog # A15544-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MEGF9 at approximately 63 kDa. The expected band size for MEGF9 is at 63 kDa.
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