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Paraformaldehyde-fixed, paraffin embedded human lung carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-MEK1/2 Polyclonal Antibody, Unconjugated (bs-1041R) at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded human lung carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-MEK1/2 Polyclonal Antibody, Unconjugated (bs-1041R) at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded human lung carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-MEK1/2 Polyclonal Antibody, Unconjugated (bs-1041R) at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining.

MEK1/2 Polyclonal Antibody

Research Use Only
BS-1041R
Bioss Antibodies
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
ReactivityBovine, Canine, Chicken, Human, Mouse, Porcine, Rabbit, Rat, Other Species
TargetMAP2K1
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    MEK1/2 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID5604
  • Target name
    MAP2K1
  • Target description
    mitogen-activated protein kinase kinase 1
  • Target synonyms
    CFC3; dual specificity mitogen-activated protein kinase kinase 1; ERK activator kinase 1; MAPK/ERK kinase 1; MAPKK 1; MAPKK1; MEK 1; MEK1; MEL; MKK1; PRKMK1; protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1)
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP36507
  • Protein Name
    Dual specificity mitogen-activated protein kinase kinase 2
  • Reactivity
    Bovine, Canine, Chicken, Human, Mouse, Porcine, Rabbit, Rat, Other Species
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Integrated Analysis of Quantitative Proteome and Transcriptional Profiles Reveals the Dynamic Function of Maternally Expressed Proteins After Parthenogenetic Activation of Buffalo Oocyte. Chen F et al., 2018 Oct, Mol Cell Proteomics
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