Western blot analysis of extracts of NIH/3T3 cells, using Phospho-MAP2K3-S189 + MAP2K6-S207 antibody (TA378251) at 1:1000 dilution.NIH/3T3 cells were treated by Anisomycin (25 ug/ml) at 37°C for 30 minutes after serum-starvation overnight. - Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. - Lysates/proteins: 25ug per lane. - Blocking buffer: 3% nonfat dry milk in TBST. - Detection: ECL Basic Kit . - Exposure time: 180s.
Western blot analysis of extracts of NIH/3T3 cells, using Phospho-MAP2K3-S189 + MAP2K6-S207 antibody (TA378251) at 1:1000 dilution.NIH/3T3 cells were treated by Anisomycin (25 ug/ml) at 37°C for 30 minutes after serum-starvation overnight. - Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. - Lysates/proteins: 25ug per lane. - Blocking buffer: 3% nonfat dry milk in TBST. - Detection: ECL Basic Kit . - Exposure time: 180s.
MEK3 (MAP2K3) Rabbit Polyclonal Antibody
TA378251
Overview
- SupplierOriGene
- Product NameMEK3 (MAP2K3) Rabbit Polyclonal Antibody
- Delivery Days Customer14
- ApplicationsWestern Blot
- CertificationResearch Use Only
- ClonalityPolyclonal
- Gene ID5606
- Target nameMAP2K3
- Target descriptionmitogen-activated protein kinase kinase 3
- Target synonymsdual specificity mitogen-activated protein kinase kinase 3; MAP kinase kinase 3; MAPK/ERK kinase 3; MAPKK 3; MAPKK3; MEK 3; MEK3; MKK3; PRKMK3; SAPK kinase 2; SAPKK2; SAPKK-2; stress-activated protein kinase kinase 2
- HostRabbit
- IsotypeIgG
- Protein IDP46734
- Protein NameDual specificity mitogen-activated protein kinase kinase 3
- Scientific DescriptionPhospho-MAP2K3-S189 + MAP2K6-S207 Rabbit polyclonal Antibody
- ReactivityHuman, Mouse
- Storage Instruction-20°C
- UNSPSC12352203