Western blot analysis of various lysates using Phospho-MAP2K4-T261 pAb (TA378256) at 1:1000 dilution. C6 cells were treated by UV at room temperature for 15-30 minutes or treated by ATP(5 mM) at 30℃ for 1 hour.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 60s.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 60s.
Western blot analysis of various lysates using Phospho-MAP2K4-T261 pAb (TA378256) at 1:1000 dilution. C6 cells were treated by UV at room temperature for 15-30 minutes or treated by ATP(5 mM) at 30℃ for 1 hour.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 60s.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 60s.
MEK4 (MAP2K4) Rabbit Polyclonal Antibody
TA378256
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetMAP2K4
Overview
- SupplierOriGene
- Product NameMEK4 (MAP2K4) Rabbit Polyclonal Antibody
- Delivery Days Customer14
- ApplicationsWestern Blot
- CertificationResearch Use Only
- ClonalityPolyclonal
- Gene ID6416
- Target nameMAP2K4
- Target descriptionmitogen-activated protein kinase kinase 4
- Target synonymsJNKK, JNKK1, MAPKK4, MEK4, MKK4, PRKMK4, SAPKK-1, SAPKK1, SEK1, SERK1, SKK1, dual specificity mitogen-activated protein kinase kinase 4, JNK-activated kinase 1, JNK-activating kinase 1, MAP kinase kinase 4, MAPK/ERK kinase 4, MAPKK 4, MEK 4, SAPK/ERK kinase 1, c-Jun N-terminal kinase kinase 1, c-Jun NH2-terminal kinase kinase 1, stress-activated protein kinase kinase 1
- HostRabbit
- IsotypeIgG
- Protein IDP45985
- Protein NameDual specificity mitogen-activated protein kinase kinase 4
- Scientific DescriptionPhospho-MAP2K4-T261 Rabbit polyclonal Antibody
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C
- UNSPSC12352203
MSDS
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