![Non-transfected (–) and transfected (+) 293T whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with SEK1 / MKK4 antibody [HL1839] (GTX637564) diluted at 1:5000. The HRP-conjated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Non-transfected (–) and transfected (+) 293T whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with SEK1 / MKK4 antibody [HL1839] (GTX637564) diluted at 1:5000. The HRP-conjated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637564/GTX637564_T-44823_20230414_WB_multiple_B_23041723_957.webp)
Non-transfected (–) and transfected (+) 293T whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with SEK1 / MKK4 antibody [HL1839] (GTX637564) diluted at 1:5000. The HRP-conjated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
SEK1 / MKK4 antibody [HL1839]
GTX637564
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetMAP2K4
Overview
- SupplierGeneTex
- Product NameSEK1 / MKK4 antibody [HL1839]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL1839
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID6416
- Target nameMAP2K4
- Target descriptionmitogen-activated protein kinase kinase 4
- Target synonymsJNKK, JNKK1, MAPKK4, MEK4, MKK4, PRKMK4, SAPKK-1, SAPKK1, SEK1, SERK1, SKK1, dual specificity mitogen-activated protein kinase kinase 4, JNK-activated kinase 1, JNK-activating kinase 1, MAP kinase kinase 4, MAPK/ERK kinase 4, MAPKK 4, MEK 4, SAPK/ERK kinase 1, c-Jun N-terminal kinase kinase 1, c-Jun NH2-terminal kinase kinase 1, stress-activated protein kinase kinase 1
- HostRabbit
- IsotypeIgG
- Protein IDP45985
- Protein NameDual specificity mitogen-activated protein kinase kinase 4
- Scientific DescriptionThis gene encodes a member of the mitogen-activated protein kinase (MAPK) family. Members of this family act as an integration point for multiple biochemical signals and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation, and development. They form a three-tiered signaling module composed of MAPKKKs, MAPKKs, and MAPKs. This protein is phosphorylated at serine and threonine residues by MAPKKKs and subsequently phosphorylates downstream MAPK targets at threonine and tyrosine residues. A similar protein in mouse has been reported to play a role in liver organogenesis. A pseudogene of this gene is located on the long arm of chromosome X. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
![Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SEK1 / MKK4 antibody [HL1839] (GTX637564) diluted at 1:1000. The HRP-conjated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SEK1 / MKK4 antibody [HL1839] (GTX637564) diluted at 1:1000. The HRP-conjated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637564/GTX637564_T-44823_20230414_WB_TPM_watermark_23041723_683.webp)
![Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SEK1 / MKK4 antibody [HL1839] (GTX637564) diluted at 1:1000. The HRP-conjated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SEK1 / MKK4 antibody [HL1839] (GTX637564) diluted at 1:1000. The HRP-conjated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637564/GTX637564_T-44823_20230414_WB_M_R_23041723_821.webp)
![SEK1 / MKK4 antibody [HL1839] detects SEK1 / MKK4 protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse adrenal gland. SEK1 / MKK4 stained by SEK1 / MKK4 antibody [HL1839] (GTX637564) diluted at 1:50. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min SEK1 / MKK4 antibody [HL1839] detects SEK1 / MKK4 protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse adrenal gland. SEK1 / MKK4 stained by SEK1 / MKK4 antibody [HL1839] (GTX637564) diluted at 1:50. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX637564/GTX637564_T-44823_20230630_IHC-P_M_23062718_930.webp)
![SEK1 / MKK4 antibody [HL1839] detects SEK1 / MKK4 protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. SEK1 / MKK4 stained by SEK1 / MKK4 antibody [HL1839] (GTX637564) diluted at 1:50. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min SEK1 / MKK4 antibody [HL1839] detects SEK1 / MKK4 protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. SEK1 / MKK4 stained by SEK1 / MKK4 antibody [HL1839] (GTX637564) diluted at 1:50. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX637564/GTX637564_T-44823_20230630_IHC-P_23062718_714.webp)
![SEK1 / MKK4 antibody [HL1839] detects SEK1 / MKK4 protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded rat urinary bladder. SEK1 / MKK4 stained by SEK1 / MKK4 antibody [HL1839] (GTX637564) diluted at 1:50. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min SEK1 / MKK4 antibody [HL1839] detects SEK1 / MKK4 protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded rat urinary bladder. SEK1 / MKK4 stained by SEK1 / MKK4 antibody [HL1839] (GTX637564) diluted at 1:50. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX637564/GTX637564_T-44823_20230630_IHC-P_R_23062718_250.webp)






