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ProductsBack/MEK4 (MAP2K4) Rabbit Polyclonal Antibody
Western blot analysis of extracts of HeLa cells, using Phospho-MAP2K4-S257/T261 antibody (TA378257) at 1:1000 dilution.HeLa cells were treated by UV at room temperature for 15-30 minutes. - Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. - Lysates/proteins: 25ug per lane. - Blocking buffer: 3% nonfat dry milk in TBST. - Detection: ECL Basic Kit . - Exposure time: 60s.
Western blot analysis of extracts of HeLa cells, using Phospho-MAP2K4-S257/T261 antibody (TA378257) at 1:1000 dilution.HeLa cells were treated by UV at room temperature for 15-30 minutes. - Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. - Lysates/proteins: 25ug per lane. - Blocking buffer: 3% nonfat dry milk in TBST. - Detection: ECL Basic Kit . - Exposure time: 60s.
Western blot analysis of extracts of HeLa cells, using Phospho-MAP2K4-S257/T261 antibody (TA378257) at 1:1000 dilution.HeLa cells were treated by UV at room temperature for 15-30 minutes. - Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. - Lysates/proteins: 25ug per lane. - Blocking buffer: 3% nonfat dry milk in TBST. - Detection: ECL Basic Kit . - Exposure time: 60s.

MEK4 (MAP2K4) Rabbit Polyclonal Antibody

TA378257
OriGene
ApplicationsWestern Blot
Product group Antibodies
TargetMAP2K4
100 ul
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Overview

  • Supplier
    OriGene
  • Product Name
    MEK4 (MAP2K4) Rabbit Polyclonal Antibody
  • Delivery Days Customer
    14
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID6416
  • Target name
    MAP2K4
  • Target description
    mitogen-activated protein kinase kinase 4
  • Target synonyms
    JNKK, JNKK1, MAPKK4, MEK4, MKK4, PRKMK4, SAPKK-1, SAPKK1, SEK1, SERK1, SKK1, dual specificity mitogen-activated protein kinase kinase 4, JNK-activated kinase 1, JNK-activating kinase 1, MAP kinase kinase 4, MAPK/ERK kinase 4, MAPKK 4, MEK 4, SAPK/ERK kinase 1, c-Jun N-terminal kinase kinase 1, c-Jun NH2-terminal kinase kinase 1, stress-activated protein kinase kinase 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP45985
  • Protein Name
    Dual specificity mitogen-activated protein kinase kinase 4
  • Scientific Description
    Phospho-MAP2K4-S257/T261 Rabbit polyclonal Antibody
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Western Blot Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate All lanes: MAP2K4 antibody at 3.25ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 45, 46 kDa Observed band size: 45 kDa
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Figure 1. Western blot analysis of MAP2K4 using anti-MAP2K4 antibody (A01725-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human placenta tissue lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: human 22RV1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAP2K4 antigen affinity purified polyclonal antibody (Catalog # A01725-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAP2K4 at approximately 44KD. The expected band size for MAP2K4 is at 44KD.
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