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Melanoma, HMB45

ME505
Nordic-MUbio
ApplicationsImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetPMEL
5 ml
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Overview

  • Supplier
    Nordic-MUbio
  • Product Name
    Melanoma, HMB45
  • Delivery Days Customer
    7
  • Application Supplier Note
    IHC(P)
  • Applications
    ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    Immunohistochemistry (paraffin);Immunohistochemistry (paraffin)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    HMB45
  • Gene ID6490
  • Target name
    PMEL
  • Target description
    premelanosome protein
  • Target synonyms
    D12S53E, HMB-45, HMB45, ME20, ME20-M, ME20M, P1, P100, PMEL17, SI, SIL, SILV, gp100, melanocyte protein PMEL, melanocyte protein Pmel 17, melanocyte protein mel 17, melanocytes lineage-specific antigen GP100, melanoma-associated ME20 antigen, melanosomal matrix protein17, silver locus protein homolog, silver, mouse, homolog of
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP40967
  • Protein Name
    Melanocyte protein PMEL
  • Reactivity Supplier Note
    Extract from lymph node metastases of melanoma
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Melanoma gp100 using anti-Melanoma gp100 antibody (A01262-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat heart tissue lysate, Lane 2: mouse heart tissue lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Melanoma gp100 antigen affinity purified polyclonal antibody (Catalog # A01262-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Melanoma gp100 at approximately 70KD. The expected band size for Melanoma gp100 is at 70KD.
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