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Anti-MELK [ARC2032] Monoclonal Antibody

CAB3530
Assay Genie
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetMELK
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Overview

  • Supplier
    Assay Genie
  • Product Name
    Anti-MELK [ARC2032] Monoclonal Antibody
  • Delivery Days Customer
    9
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    WB
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    ARC2032
  • Conjugate
    Unconjugated
  • Gene ID9833
  • Target name
    MELK
  • Target description
    maternal embryonic leucine zipper kinase
  • Target synonyms
    HPK38, maternal embryonic leucine zipper kinase, pEg3 kinase, protein kinase Eg3, protein kinase PK38, tyrosine-protein kinase MELK
  • Host
    Rabbit
  • Protein IDQ14680
  • Protein Name
    Maternal embryonic leucine zipper kinase
  • Scientific Description
    MELK Monoclonal Antibody is a premium monoclonal antibody that offers outstanding performance and reliability for demanding research applications. Rigorously validated for WB, ELISA, this antibody ensures consistent, reproducible results across multiple experimental platforms. Demonstrates excellent reactivity with Human,Mouse,Rat samples, providing researchers with confidence in cross-species compatibility. Conveniently packaged in 100microL format to meet your experimental needs. For optimal performance, store at -20°C and maintains stability for 12 months. Backed by rigorous quality control testing to ensure superior performance in your critical research applications.
  • Shelf life instruction
    12 months
  • Source
    Rabbit
  • Reactivity
    Human
  • Reactivity Supplier
    Human,Mouse,Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of MELK using anti-MELK antibody (A04038-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human U87 whole cell lysates, Lane 5: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MELK antigen affinity purified polyclonal antibody (Catalog # A04038-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MELK at approximately 66 kDa. The expected band size for MELK is at 75 kDa.
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