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Western Blot analysis of HuvEc cells using MerTK/Tyro3 Polyclonal Antibody
Western Blot analysis of HuvEc cells using MerTK/Tyro3 Polyclonal Antibody
Western Blot analysis of HuvEc cells using MerTK/Tyro3 Polyclonal Antibody

MERTK/TYRO3 Antibody

CSB-PA003230
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetTYRO3
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Overview

  • Supplier
    Cusabio
  • Product Name
    MERTK/TYRO3 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID7301
  • Target name
    TYRO3
  • Target description
    TYRO3 protein tyrosine kinase
  • Target synonyms
    BYK, Dtk, Etk-2, RSE, Rek, Sky, Tif, tyrosine-protein kinase receptor TYRO3, tyrosine-protein kinase DTK, tyrosine-protein kinase RSE, tyrosine-protein kinase SKY, tyrosine-protein kinase TIF, tyrosine-protein kinase byk
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ06418
  • Protein Name
    Tyrosine-protein kinase receptor TYRO3
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of TYRO3 using anti-TYRO3 antibody (M00913-5). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TYRO3 antigen affinity purified monoclonal antibody (Catalog # M00913-5) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TYRO3 at approximately 120 kDa. The expected band size for TYRO3 is at 97 kDa.
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