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ProductsBack/METAP2 Antibody (AP)

METAP2 Antibody (AP)

LS-C408900
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetMETAP2
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    METAP2 Antibody (AP)
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 50kDa/52kDa, while the observed MW by Western blot was 60kDa.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:200 - 1:2000) The predicted MW is 50kDa/52kDa, while the observed MW by Western blot was 60kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Alk. Phosphatase
  • Estimated Purity
    ...
  • Gene ID10988
  • Target name
    METAP2
  • Target description
    methionyl aminopeptidase 2
  • Target synonyms
    eIF-2-associated p67 homolog; initiation factor 2-associated 67 kDa glycoprotein; MAP2; methionine aminopeptidase 2; MNPEP; p67eIF2; peptidase M 2; testicular tissue protein Li 17
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of METAP2 using anti-METAP2 antibody (A03648-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: human DLD-1 whole cell lysates, Lane 6: human HT1080 whole cell lysates, Lane 7: human HL-60 whole cell lysates, Lane 8: monkey COS-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-METAP2 antigen affinity purified polyclonal antibody (Catalog # A03648-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for METAP2 at approximately 67 kDa. The expected band size for METAP2 is at 53 kDa.
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