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Anti-METAP2 [ARC2022] Monoclonal Antibody

CAB3465
Assay Genie
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetMETAP2
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Overview

  • Supplier
    Assay Genie
  • Product Name
    Anti-METAP2 [ARC2022] Monoclonal Antibody
  • Delivery Days Customer
    9
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    WB
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    ARC2022
  • Conjugate
    Unconjugated
  • Gene ID10988
  • Target name
    METAP2
  • Target description
    methionyl aminopeptidase 2
  • Target synonyms
    MAP2, MNPEP, p67eIF2, methionine aminopeptidase 2, eIF-2-associated p67 homolog, initiation factor 2-associated 67 kDa glycoprotein, peptidase M 2, testicular tissue protein Li 17
  • Host
    Rabbit
  • Protein IDP50579
  • Protein Name
    Methionine aminopeptidase 2
  • Scientific Description
    METAP2 Monoclonal Antibody is a premium monoclonal antibody that offers outstanding performance and reliability for demanding research applications. Rigorously validated for WB, ELISA, this antibody ensures consistent, reproducible results across multiple experimental platforms. Demonstrates excellent reactivity with Human,Mouse,Rat samples, providing researchers with confidence in cross-species compatibility. Conveniently packaged in 100microL format to meet your experimental needs. For optimal performance, store at -20°C and maintains stability for 12 months. Backed by rigorous quality control testing to ensure superior performance in your critical research applications.
  • Shelf life instruction
    12 months
  • Source
    Rabbit
  • Reactivity
    Human
  • Reactivity Supplier
    Human,Mouse,Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of METAP2 using anti-METAP2 antibody (A03648-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: human DLD-1 whole cell lysates, Lane 6: human HT1080 whole cell lysates, Lane 7: human HL-60 whole cell lysates, Lane 8: monkey COS-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-METAP2 antigen affinity purified polyclonal antibody (Catalog # A03648-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for METAP2 at approximately 67 kDa. The expected band size for METAP2 is at 53 kDa.
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