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Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA013748LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA013748LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA013748LA01HU at dilution of 1:100

MFAP3L Antibody

CSB-PA013748LA01HU
Cusabio
ApplicationsImmunoFluorescence, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetMFAP3L
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Overview

  • Supplier
    Cusabio
  • Product Name
    MFAP3L Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID9848
  • Target name
    MFAP3L
  • Target description
    microfibril associated protein 3 like
  • Target synonyms
    NYD-sp9, microfibrillar-associated protein 3-like, microfi brillar-associated protein 3-like, microfibrillar associated protein 3 like, testis development protein NYD-SP9
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO75121
  • Protein Name
    Microfibrillar-associated protein 3-like
  • Scientific Description
    cytoplasm, nucleus, plasma membrane
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of MFAP3L using anti-MFAP3L antibody (A12581). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: rat testis tissue lysates, Lane 5: rat PC-12 whole cell lysates, Lane 6: mouse testis tissue lysates, Lane 7: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MFAP3L antigen affinity purified polyclonal antibody (Catalog # A12581) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MFAP3L at approximately 45 kDa. The expected band size for MFAP3L is at 45 kDa.
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