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Mgat4B Polyclonal Antibody

CAC11570
Biomatik
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetMGAT4B
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Overview

  • Supplier
    Biomatik
  • Product Name
    Mgat4B Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA, WB, IHC; Recommended dilution: WB:1:500-1:5000, IHC:1:200-1:500
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID11282
  • Target name
    MGAT4B
  • Target description
    alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase B
  • Target synonyms
    GNT-IV, GNT-IVB, alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase B, N-acetylglucosaminyltransferase IVb, N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase IVb, UDP-N-acetylglucosamine: alpha-1,3-D-mannoside beta-1,4-N-acetylglucosaminyltransferase IV, UDP-N-acetylglucosamine: alpha-1,3-D-mannoside beta-1,4-N-acetylglucosaminyltransferase IVb, alpha-1,3-mannosyl-glycoprotein beta-1,4-N-acetylglucosaminyltransferase, aminyltransferase, glcNAc-T IVb, mannosyl (alpha-1,3-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase, isoenzyme B, mannosyl (alpha-1,3-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase, isozyme B
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UQ53
  • Protein Name
    Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase B
  • Scientific Description
    The Mgat4B Polyclonal Antibody (Species: Human) has been validated for the following applications: ELISA, WB, IHC.
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MGAT4B using anti-MGAT4B antibody (A10339). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human RT4 whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: human Caco-2 whole cell lysates, Lane 5: rat kidney tissue lysates, Lane 6: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MGAT4B antigen affinity purified polyclonal antibody (Catalog # A10339) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MGAT4B at approximately 70 kDa. The expected band size for MGAT4B is at 63 kDa.
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