
FACS analysis of HeLa cells using GTX01028 MGEA5 antibody. Green : Primary antibdoy Blue : Isotype control Dilution : 1microg/1x10? cells Fixation : 2% paraformaldehyde (10 min) Permeabilization : 90% methanol for 10 min
MGEA5 antibody
GTX01028
ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetOGA
Overview
- SupplierGeneTex
- Product NameMGEA5 antibody
- Delivery Days Customer9
- Application Supplier NoteWB: 1:2000. IHC-P: 1:25. FACS: 1:25. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- ConjugateUnconjugated
- Gene ID10724
- Target nameOGA
- Target descriptionO-GlcNAcase
- Target synonymsbeta-hexosaminidase; beta-N-acetylglucosaminidase; beta-N-acetylhexosaminidase; bifunctional protein NCOAT; hyaluronidase in meningioma; MEA5; meningioma expressed antigen 5 (hyaluronidase); meningioma-expressed antigen 5; MGEA5; N-acetyl-beta-D-glucosaminidase; N-acetyl-beta-glucosaminidase; NCOAT; nuclear cytoplasmic O-GlcNAcase and acetyltransferase; protein O-GlcNAcase
- HostRabbit
- IsotypeIgG
- Protein IDO60502
- Protein NameProtein O-GlcNAcase
- Scientific DescriptionThe dynamic modification of cytoplasmic and nuclear proteins by O-linked N-acetylglucosamine (O-GlcNAc) addition and removal on serine and threonine residues is catalyzed by OGT (MIM 300255), which adds O-GlcNAc, and MGEA5, a glycosidase that removes O-GlcNAc modifications (Gao et al., 2001 [PubMed 11148210]).[supplied by OMIM, Mar 2008]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203