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Western blot All lanes: MGME1 antibody at 1ug/ml Lane 1: Jurkat whole cell lysate Lane 2: 293T whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 39 kDa Observed band size: 39 kDa
Western blot All lanes: MGME1 antibody at 1ug/ml Lane 1: Jurkat whole cell lysate Lane 2: 293T whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 39 kDa Observed band size: 39 kDa
Western blot All lanes: MGME1 antibody at 1ug/ml Lane 1: Jurkat whole cell lysate Lane 2: 293T whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 39 kDa Observed band size: 39 kDa

MGME1 Antibody

CSB-PA880084LA01HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetMGME1
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Overview

  • Supplier
    Cusabio
  • Product Name
    MGME1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID92667
  • Target name
    MGME1
  • Target description
    mitochondrial genome maintenance exonuclease 1
  • Target synonyms
    bA504H3.4; C20orf72; DDK1; mitochondrial genome maintenance exonuclease 1; MTDPS11
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9BQP7
  • Protein Name
    Mitochondrial genome maintenance exonuclease 1
  • Scientific Description
    Metal-dependent single-stranded DNA (ssDNA) exonuclease involved in mitochondrial genome maintenance. Has preference for 5-3 exonuclease activity but is also capable of endoduclease activity on linear substrates. Necessary for maintenance of proper 7S DNA levels. Probably involved in mitochondrial DNA (mtDNA) repair, possibly via the processing of displaced DNA containing Okazaki fragments during RNA-primed DNA synthesis on the lagging strand or via processing of DNA flaps during long-patch base excision repair. Specifically binds 5-hydroxymethylcytosine (5hmC)-containing DNA in stem cells.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MGME1 using anti-MGME1 antibody (A09360-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MGME1 antigen affinity purified polyclonal antibody (Catalog # A09360-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MGME1 at approximately 36 kDa. The expected band size for MGME1 is at 36 kDa.
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