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ProductsBack/MICA/B Mouse Monoclonal Antibody [Clone ID: BAMO1]
Western blot analysis of MICA/B expression in 293T (1), Jurkat (2), HeLa (3) and DLD-1 (4) using AM26694AF-N.
Western blot analysis of MICA/B expression in 293T (1), Jurkat (2), HeLa (3) and DLD-1 (4) using AM26694AF-N.
Western blot analysis of MICA/B expression in 293T (1), Jurkat (2), HeLa (3) and DLD-1 (4) using AM26694AF-N.

MICA/B Mouse Monoclonal Antibody [Clone ID: BAMO1]

AM26694AF-N
OriGene
ApplicationsFlow Cytometry, Western Blot
Product group Antibodies
ReactivityHuman
TargetMICA
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    MICA/B Mouse Monoclonal Antibody [Clone ID: BAMO1]
  • Delivery Days Customer
    14
  • Applications
    Flow Cytometry, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    BAMO1
  • Gene ID100507436
  • Target name
    MICA
  • Target description
    MHC class I polypeptide-related sequence A
  • Target synonyms
    MIC-A, PERB11.1, MHC class I polypeptide-related sequence A, HLA class I antigen, MHC class I chain-related protein A, MHC class I related chain A, MHC class I related sequence A, major histocompatibility complex class I chain-related protein A, stress inducible class I homolog
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ29983
  • Protein Name
    MHC class I polypeptide-related sequence A
  • Scientific Description
    MICA/B mouse monoclonal antibody, clone BAMO1, Azide Free
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of MICA using anti-MICA antibody (PB9612). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates, Lane 2: human SGC-7901 whole cell lysates, Lane 3: human SW620 whole cell lysates, Lane 4: huamn Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MICA antigen affinity purified polyclonal antibody (Catalog # PB9612) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MICA at approximately 45 kDa. The expected band size for MICA is at 43 kDa.
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