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ProductsBack/MIOX Mouse Monoclonal Antibody [Clone ID: OTI2F4]
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MIOX (RC210070, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MIOX. Positive lysates LY413679 (100ug) and LC413679 (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MIOX (RC210070, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MIOX. Positive lysates LY413679 (100ug) and LC413679 (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MIOX (RC210070, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MIOX. Positive lysates LY413679 (100ug) and LC413679 (20ug) can be purchased separately from OriGene.

MIOX Mouse Monoclonal Antibody [Clone ID: OTI2F4]

CF501423
OriGene
ApplicationsFlow Cytometry, Western Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetMIOX
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    MIOX Mouse Monoclonal Antibody [Clone ID: OTI2F4]
  • Delivery Days Customer
    14
  • Applications
    Flow Cytometry, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI2F4
  • Gene ID55586
  • Target name
    MIOX
  • Target description
    myo-inositol oxygenase
  • Target synonyms
    ALDRL6, inositol oxygenase, MI oxygenase, aldehyde reductase (aldose reductase) like 6, aldehyde reductase-like 6, kidney-specific protein 32, renal-specific oxidoreductase
  • Host
    Mouse
  • Isotype
    IgG2b
  • Protein IDQ9UGB7
  • Protein Name
    Inositol oxygenase
  • Scientific Description
    Carrier-free (BSA/glycerol-free) MIOX mouse monoclonal antibody, clone OTI2F4 (formerly 2F4)
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MIOX using anti-MIOX antibody (A06957). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MIOX antigen affinity purified polyclonal antibody (Catalog # A06957) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MIOX at approximately 36 kDa. The expected band size for MIOX is at 33 kDa.
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TargetMIOX
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Immunohistochemistry analysis in human kidney and pancreas tissues using Anti-MIOX antibody. Corresponding MIOX RNA-seq data are presented for the same tissues.
Product group Antibodies
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Product group Antibodies
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TargetMIOX
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Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with MIOX antibody (GTX116239) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Product group Antibodies
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