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ProductsBack/MIRO2 (RHOT2) Rabbit Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using (TA380922) at 1:1000 dilution. - Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. - Lysates/proteins: 25ug per lane. - Blocking buffer: 3% nonfat dry milk in TBST. - Detection: ECL Basic Kit . - Exposure time: 30s.
Western blot analysis of extracts of various cell lines, using (TA380922) at 1:1000 dilution. - Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. - Lysates/proteins: 25ug per lane. - Blocking buffer: 3% nonfat dry milk in TBST. - Detection: ECL Basic Kit . - Exposure time: 30s.
Western blot analysis of extracts of various cell lines, using (TA380922) at 1:1000 dilution. - Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. - Lysates/proteins: 25ug per lane. - Blocking buffer: 3% nonfat dry milk in TBST. - Detection: ECL Basic Kit . - Exposure time: 30s.

MIRO2 (RHOT2) Rabbit Polyclonal Antibody

TA380922
OriGene
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
TargetRHOT2
100 ul
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Overview

  • Supplier
    OriGene
  • Product Name
    MIRO2 (RHOT2) Rabbit Polyclonal Antibody
  • Delivery Days Customer
    14
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID89941
  • Target name
    RHOT2
  • Target description
    ras homolog family member T2
  • Target synonyms
    ARHT2; C16orf39; MIRO2; MIRO-2; mitochondrial Rho (MIRO) GTPase 2; mitochondrial Rho GTPase 2; ras homolog gene family, member T2; RASL
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8IXI1
  • Protein Name
    Mitochondrial Rho GTPase 2
  • Scientific Description
    RHOT2 Rabbit polyclonal Antibody
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of MIRO2/RHOT2 using anti-MIRO2/RHOT2 antibody (A08801-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MIRO2/RHOT2 antigen affinity purified polyclonal antibody (A08801-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MIRO2/RHOT2 at approximately 80 kDa. The expected band size for MIRO2/RHOT2 is at 68 kDa.
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