ICC/IF analysis of mouse melanoma B16 cells using GTX00738 MITF antibody. Green : Primary antibody Blue : DAPI Dilution : 1:1000 Fixation : 4% PFA Permeabilization : 0.25 % Triton X-100
ICC/IF analysis of mouse melanoma B16 cells using GTX00738 MITF antibody. Green : Primary antibody Blue : DAPI Dilution : 1:1000 Fixation : 4% PFA Permeabilization : 0.25 % Triton X-100
MITF antibody
GTX00738
ApplicationsImmunoFluorescence, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetMITF
Overview
- SupplierGeneTex
- Product NameMITF antibody - KO/KD Validated
- Delivery Days Customer9
- Application Supplier NoteWB: 1:1000-1:3000. IHC-P: 1:300-1:1000. IHC-Fr: 1:300-1:1000. ChIP assay: 1:200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- ConjugateUnconjugated
- Gene ID4286
- Target nameMITF
- Target descriptionmelanocyte inducing transcription factor
- Target synonymsbHLHe32; class E basic helix-loop-helix protein 32; CMM8; COMMAD; melanogenesis associated transcription factor; MI; microphtalmia-associated transcription factor; microphthalmia-associated transcription factor; MITF-A; WS2; WS2A
- HostRabbit
- IsotypeIgG
- Protein IDO75030
- Protein NameMicrophthalmia-associated transcription factor
- Scientific DescriptionThe protein encoded by this gene is a transcription factor that contains both basic helix-loop-helix and leucine zipper structural features. The encoded protein regulates melanocyte development and is responsible for pigment cell-specific transcription of the melanogenesis enzyme genes. Heterozygous mutations in the this gene cause auditory-pigmentary syndromes, such as Waardenburg syndrome type 2 and Tietz syndrome. [provided by RefSeq, Aug 2017]
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- The in vitro and in vivo depigmenting activity of pterostilbene through induction of autophagy in melanocytes and inhibition of UVA-irradiated alpha-MSH in keratinocytes via Nrf2-mediated antioxidant pathways. Hseu YC et al., 2021 Aug, Redox BiolRead more
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