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MKK3 (Phospho-T222) polyclonal antibody

ORB669890
Biorbyt
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetMAP2K3
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Overview

  • Supplier
    Biorbyt
  • Product Name
    MKK3 (Phospho-T222) polyclonal antibody
  • Delivery Days Customer
    10
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB: 1:500-1:1000, IHC-P: 1:50-1:200 IF, IHC, WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID5606
  • Target name
    MAP2K3
  • Target description
    mitogen-activated protein kinase kinase 3
  • Target synonyms
    MAPKK3, MEK3, MKK3, PRKMK3, SAPKK-2, SAPKK2, dual specificity mitogen-activated protein kinase kinase 3, MAP kinase kinase 3, MAPK/ERK kinase 3, MAPKK 3, MEK 3, SAPK kinase 2, stress-activated protein kinase kinase 2
  • Host
    Rabbit
  • Protein IDP46734
  • Protein Name
    Dual specificity mitogen-activated protein kinase kinase 3
  • Scientific Description
    Rabbit polyclonal antibody to MKK3 (Phospho-T222)
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MEK3 using anti-MEK3 antibody (PB9763). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: rat kidney tissue lysates, Lane 3: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MEK3 antigen affinity purified polyclonal antibody (Catalog # PB9763) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MEK3 at approximately 40 kDa. The expected band size for MEK3 is at 40 kDa.
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