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WB analysis of various sample lysates using GTX64556 MKL1 antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX64556 MKL1 antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX64556 MKL1 antibody. Dilution : 1:1000 Loading : 25microg per lane

MKL1 antibody

GTX64556
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetMRTFA
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Overview

  • Supplier
    GeneTex
  • Product Name
    MKL1 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500 - 1:2000. IHC-P: 1:50 - 1:200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID57591
  • Target name
    MRTFA
  • Target description
    myocardin related transcription factor A
  • Target synonyms
    basic, SAP and coiled-coil domain; BSAC; MAL; megakaryoblastic leukemia (translocation) 1; megakaryoblastic leukemia 1 protein; megakaryocytic acute leukemia protein; MKL; MKL/myocardin-like protein 1; MKL1; MRTF-A; myocardin-related transcription factor A
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ969V6
  • Protein Name
    MKL/myocardin-like protein 1
  • Scientific Description
    The protein encoded by this gene interacts with the transcription factor myocardin, a key regulator of smooth muscle cell differentiation. The encoded protein is predominantly nuclear and may help transduce signals from the cytoskeleton to the nucleus. This gene is involved in a specific translocation event that creates a fusion of this gene and the RNA-binding motif protein-15 gene. This translocation has been associated with acute megakaryocytic leukemia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Mkl1/MRTFA using anti-Mkl1/MRTFA antibody (A32468-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Mkl1/MRTFA antigen affinity purified polyclonal antibody (Catalog # A32468-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Mkl1/MRTFA at approximately 145 kDa. The expected band size for Mkl1/MRTFA is at 145 kDa.
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