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MLC2 (Phospho-Tyr118) Antibody

ORB251690
Biorbyt
ApplicationsImmunoFluorescence, Western Blot
Product group Antibodies
ReactivityHuman
TargetMYL2
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Overview

  • Supplier
    Biorbyt
  • Product Name
    MLC2 (Phospho-Tyr118) Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot
  • Applications Supplier
    IF, WB
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Gene ID4633
  • Target name
    MYL2
  • Target description
    myosin light chain 2
  • Target synonyms
    CMH10, MFM12, MLC-2, MLC-2s/v, MLC-2v, MLC2, myosin regulatory light chain 2, ventricular/cardiac muscle isoform, RLC of myosin, S15D myosin regulatory light chain 2, T160D myosin regulatory light chain 2, cardiac myosin light chain 2, cardiac ventricular myosin light chain 2, myosin, light chain 2, regulatory, cardiac, slow, myosin, light polypeptide 2, regulatory, cardiac, slow, regulatory light chain of myosin, slow cardiac myosin regulatory light chain 2, ventricular myosin light chain 2
  • Host
    Rabbit
  • Protein IDP10916
  • Protein Name
    Myosin regulatory light chain 2, ventricular/cardiac muscle isoform
  • Scientific Description
    MLC2 (Phospho-Tyr118) Antibody
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Myosin Light Chain 2/MLC-2V/MYL2 using anti-Myosin Light Chain 2/MLC-2V/MYL2 antibody (A03215-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U20S whole cell lysates, Lane 2: monkey heart tissue lysates, Lane 3: rat heart tissue lysates, Lane 4: rat skeletal muscle tissue lysates, Lane 5: mouse heart tissue lysates, Lane 6: mouse skeletal muscle lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Myosin Light Chain 2/MLC-2V/MYL2 antigen affinity purified polyclonal antibody (Catalog # A03215-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Myosin Light Chain 2/MLC-2V/MYL2 at approximately 19 kDa. The expected band size for Myosin Light Chain 2/MLC-2V/MYL2 is at 19 kDa.
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