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MLH1 Antibody

LS-C813052
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetMLH1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    MLH1 Antibody
  • Delivery Days Customer
    14
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    ELISA (1:10000 - 1:20000), WB (1:500 - 1:2000) Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID4292
  • Target name
    MLH1
  • Target description
    mutL homolog 1
  • Target synonyms
    COCA2; DNA mismatch repair protein Mlh1; FCC2; hMLH1; HNPCC; HNPCC2; MMRCS1; mutL homolog 1, colon cancer, nonpolyposis type 2
  • Host
    Mouse
  • Isotype
    IgG2b
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MLH1 using anti-MLH1 antibody (A00126-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Daudi whole cell lysates, Lane 2: human T-47D whole cell lysates, Lane 3: human MOLT-4 whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: human U251 whole cell lysates, Lane 6: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MLH1 antigen affinity purified polyclonal antibody (Catalog # A00126-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MLH1 at approximately 85 kDa. The expected band size for MLH1 is at 85 kDa.
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