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ProductsBack/MLH1 antibody [N1N3]
MLH1 antibody detects MLH1 protein by western blot analysis. Non-transfected (-) and MLH1-transfected (+, ) 293T whole cell extracts (5 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MLH1 antibody (GTX112984) at a dilution of 1:1000.
MLH1 antibody detects MLH1 protein by western blot analysis. Non-transfected (-) and MLH1-transfected (+, ) 293T whole cell extracts (5 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MLH1 antibody (GTX112984) at a dilution of 1:1000.
MLH1 antibody detects MLH1 protein by western blot analysis. Non-transfected (-) and MLH1-transfected (+, ) 293T whole cell extracts (5 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MLH1 antibody (GTX112984) at a dilution of 1:1000.

MLH1 antibody [N1N3]

GTX112984
GeneTex
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetMLH1
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Overview

  • Supplier
    GeneTex
  • Product Name
    MLH1 antibody [N1N3]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.83 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID4292
  • Target name
    MLH1
  • Target description
    mutL homolog 1
  • Target synonyms
    COCA2, FCC2, HNPCC, HNPCC2, LYNCH2, MLH-1, MMRCS1, hMLH1, DNA mismatch repair protein Mlh1, mutL homolog 1, colon cancer, nonpolyposis type 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP40692
  • Protein Name
    DNA mismatch repair protein Mlh1
  • Scientific Description
    This gene was identified as a locus frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). It is a human homolog of the E. coli DNA mismatch repair gene mutL, consistent with the characteristic alterations in microsatellite sequences (RER+ phenotype) found in HNPCC. Alternatively spliced transcript variants encoding different isoforms have been described, but their full-length natures have not been determined. [provided by RefSeq]
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of MLH1 using anti-MLH1 antibody (A00126-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Daudi whole cell lysates, Lane 2: human T-47D whole cell lysates, Lane 3: human MOLT-4 whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: human U251 whole cell lysates, Lane 6: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MLH1 antigen affinity purified polyclonal antibody (Catalog # A00126-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MLH1 at approximately 85 kDa. The expected band size for MLH1 is at 85 kDa.
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